http://www.accessexcellence.org/AB/GG/plasmid.html

National health Museum About Biotech website – good for graphics- cloning of plasmid DNA. Links into other areas of Genetics – wide range of topics.

http://members.aol.com/BearFlag45/Biology1A/LectureNotes/lec19.html#anchor1491876

Transcription and Translation – useful for RNA – introns and exons – eukaryote gene expression.

http://members.aol.com/BearFlag45/Biology1A/LectureNotes/lec23.html

Recombinant DNA Technology lecture notes and colour graphics

http://darwin.nmsu.edu/~molbio/mcb520/lecture2.html

Restriction Enzymes – DNA Technology and useful for introduction.

http://www.brinkmann.com/PCR_appl_primer.asp

http://www.blc.arizona.edu/courses/181gh/rick/expression2/intro.html

Eukaryotic genome

http://www.dur.ac.uk/biological.sciences/Staff/Croy/cDNAfigs.htm

cDNA synthesis

http://allserv.rug.ac.be/~avierstr/principles/pcr.html

http://www.faseb.org/opa/bloodsupply/pcr.html

PCR

http://www.brinkmann.com/PCR_appl_primer.asp

http://allserv.rug.ac.be/~avierstr/principles/pcr.html

http://www.faseb.org/opa/bloodsupply/pcr.html

Web.mit.edu/esgbio/www/rdna/pcr.html

There are many good sites that discuss site specific mutagenesis
http://biology.ecsu.ctstateu.edu/courses/molgen/ directed%20mutagen/SiteDiremut.html
http:// www.moorhead.msus.edu/chastain/html/mut.html
http:// www.nobel.se/chemistry/laureates/1993/illpres/site.html

You can model amino acid substitutions with expasy http://www.expasy.ch/swissmod/SWISS- MODEL.html
You can construct ligands with InsightII or Sybyl or CAChe (they have a free demo) http://www.cachesoftware.com/
You can dock ligands with mutated enzyme structures using these programs or use DOCK and score your matches. Information on DOCK can be found at http:// www.cmpharm.ucsf.edu/kuntz/dock.html

www.madsci.org/posts/archives/oct2001/1004108157.Mb.r.html

www.people.virginia.edu/~rjh9u/pcranim.html - good animation

www.wellcome.ac.uk/en/genome/technologies/hg17b009.html

MadSci Network: Molecular Biology

http://www.ornl.gov/TechResources/Human_Genome/publicat/hgn/v9n3/02doenih.html

http://www.science.doe.gov/grants/lab99_04.html

http://ocw.mit.edu/NR/rdonlyres/Biology/7-02Introduction-to-Experimental-BiologyFall2001/26BD18EA-A4ED-4E61-86F4-4B40F346C364/0/fa01recrdm1.pdf

http://www.genome.ou.edu/protocol_book/protocol_index.html

List of protocols of techniques – agarose gel electrophoresis, DNA elution etc.

http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/recomb_DNA.html

very good for questions and interaction eg design of plasmid vector

http://www.accessexcellence.org/AB/IE/Speaking_Language_rDNA.html

Text good – useful diagrams – not very exciting – no animation or inte

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/Transcription.html.

GOOD INFO AND DIAGRAMS.

http://open-site.org/Science/Biology/Molecular_Biology/.

WORDS BUT QUITE BASIC.

http://www.indstate.edu/thcme/mwking/rna.html.

http://www.blc.arizona.edu/courses/181gh/rick/expression2/intro.html.

SOME INFO BELOW- OTHER GOOD LINKS ALSO ON ABOVE ADDRESS.

MORE ADDRESSES.

SYNTHESIS OF DNA BELOW

http://www.dur.ac.uk/biological.sciences/Staff/Croy/cDNAfigs.htm

http://www.cbs.umn.edu/~kclark/protocols/library.html.

CDNA LIBRARIES ABOVE GOOD LINK.

http://www.roche-applied-science.com/fst/magnapure.htm?/sis/magnapure/techniques/techniques_03.htm.

MAGNA BEADS ABOVE.

GOOD GRAPHICS AND INFO.

Techniques involving RNA and DNA

http://www.biology.ewu.edu/aHerr/Genetics/Bio310/Pages/ch21pges/LN14final.pdf

(The eukaryotic genome and its expression.)

http://www.dynalbiotech.com/kunder/dynal/DynalPub401.nsf/frames/index.html?open&6=/$all/844209E648A03BCBC1256C5400488DDD

(isolation of mRNA, construction of cDNA libraries)

Diagram of cDNA library

http://www.brad.ac.uk/staff/smpicksley/cDNA%20lecture_files/v3_document.htm

isolation of mRNA and synthesis of cDNA (slide format)

http://www.metachem.co.uk/BNmRNA.htm

isolation of mRNA protocol

http://www.dcs.shef.ac.uk/~neil/BioART/libraryFaq.htm

Question for cDNA libraries

http://www.evrogen.com/t6.shtml

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html#Transforming_E._coli Good info and diagrams

http://web.mit.edu/esgbio/www/rdna/cloning.html Cloning insulin using Ecoli, good diagrams

http://www.accessexcellence.org/AB/GG/plasmid.html good picture and abstract (cloning by plasmid vector)

http://www.bhsu.edu/artssciences/chemistry/Biochem/Lecture_Notes/Chapter29.pdf lots of pages too many

http://members.aol.com/BearFlag45/Biology1A/LectureNotes/lec19.html#anchor1491876 (introns & exons + good diagrams)

http://members.aol.com/BearFlag45/Biology1A/LectureNotes/lec23.html

(genomic library)

http://darwin.nmsu.edu/~molbio/mcb520/lecture2.html (cloning vectors + other recombinant DNA info)WEBSITES

http://users.rcn.com/ /BiologyPages/R/RecombinantDNA.html

GOOD PICTURES

http://www.geocities.com/SiliconValley/5504/biochem.html

http://homepages.uel.ac.uk/V.K.Sieber/dna.htm

http://hdklab.wustl.edu/lab_manual/plasmid/plsmid10.html

DESCRIBES THE PROCESS OF PLASMID SUBCLONING CONTAINS DIAGRAM

http://hsc.virginia.edu/research/UVA-Guidelines.html

http://www.evrogen.com/s3.shtml

WWW.PROMEGA.COM/APPLICATION/RT-CDNA/DEFAULT

http://www.blc.arizona.edu/courses/181gh/rick/expression2/intro.html